Application

Research CategoryStem Cell Research

Research Sub CategoryMesenchymal Stem Cells

A separate ECM solution is not required to facilitate cell attachment using the human mesenchymal-XF expansion medium.

Components

All components used within the media are of human origin and do not contain any animal derived components.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Human Mesenchymal-XF Expansion Medium is a xeno-free medium specially formulated for the rapid expansion of human mesenchymal stem cells isolated from a variety of sources, including adipose and bone marrow tissues. The medium contains 2% human serum, is free of animal-derived components and supports the growth of human MSCs at rates equal to or greater than other commercial xeno-free/serum-free media and media supplemented with 2-10% FBS. Human MSCs cultured in the medium retain self-renewal and multi-lineage differenitation potential. The Human Mesenchymal-XF Expansion Medium does not contain any antimicrobials, phenol red, or any other components that can cause cell stress and “masking effects” that may influence experimental results.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

The Human Mesenchymal-XF Expansion Medium is a complete medium and contains all the necessary growth factors and supplements to support human mesenchymal stem cell growth.

Quality

These products are manufactured with the highest quality of raw materials, with exacting standards and production procedures to ensure lot-to-lot consistency. Every lot of medium is extensively tested for the following parameters:

Sterility Testing: Negative for bacterial and fungal growth
pH: 7.5 ± 0.3
Cell Testing: Rate of proliferation and morphology
Osmolality: 255 ± 15 mOsm
Endotoxin: ﹤0.5 EU/mL

Storage and Stability

Frozen medium should be stored at -20°C until expiration date.
Thawed medium can be stored at 2 to 8°C for up to 1 month.
Multiple freeze/thaw cycles are not recommended